human colorectal cancer crc cell lines hct116  (ATCC)

Journal: Oncotarget

Article Title: miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4

doi: 10.18632/oncotarget.19495

Figure Lengend Snippet: (A) Analyses of miR-543 expression after transfection in HCT116 and SW-480 cells by real-time PCR. (B, C) Influence of miR-543 downregulation on cell proliferation of HCT116 and SW-480 cells by CCK-8 (B) and EdU assays. (C, D) Influence of miR-543 knockdown on cell apoptosis. * p <0.05, Scale bar = 100 μm for (C); the data represent the mean ± SD from triplicate measurements.

Article Snippet: Human CRC cancer cells lines HCT116, SW-480 and HT29 were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Transfection, Real-time Polymerase Chain Reaction, CCK-8 Assay, Knockdown

Journal: Oncotarget

Article Title: miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4

doi: 10.18632/oncotarget.19495

Figure Lengend Snippet: (A) Effect of miR-543 knockdown on cell migration and invasion ability in HCT116 and SW-480 cells, Scale bar = 50 μm. (B) E-cadherin and vimentin expression in HCT116 and SW-480 cells by western blot. (C) Epithelial-to-mesenchymal transition (EMT)-associated genes expression in HCT116 and SW-480 cells by RT-qPCR. * p < 0.05; the data represent the mean ± SD from triplicate measurements.

Article Snippet: Human CRC cancer cells lines HCT116, SW-480 and HT29 were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Knockdown, Migration, Expressing, Western Blot, Quantitative RT-PCR

Journal: Oncotarget

Article Title: miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4

doi: 10.18632/oncotarget.19495

Figure Lengend Snippet: (A) Western blot analysis of KLF4 protein expression after transfection in HCT116 and SW-480 cells. (B) Luciferase activities of wild-type and the mutant pmirGLO- KLF4 -3′-UTR reporter in HCT116 and SW-480 cells. (C) The predicted miR-543 binding site on the KLF4 mRNA 3′-UTR and the corresponding mutations in 3′-UTR of KLF4 . (D, E) KLF4 expression on protein level (D) and mRNA level (E) was determined in CRC tissues (T) and adjacent nontumorous tissues (ANT). (F) Spearman's correlation analysis was performed to detect the association between the expression level of miR-543 and KLF4 in GC tissues. (G, H) KLF4 expression on protein level (G) and mRNA level (H) was determined in three CRC cell lines (HCT116, SW-480 and HT29) and nontumorous mucosa. Error bars represent mean ± SD from three independent experiments. * p < 0.05.

Article Snippet: Human CRC cancer cells lines HCT116, SW-480 and HT29 were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Western Blot, Expressing, Transfection, Luciferase, Mutagenesis, Binding Assay

Journal: Oncotarget

Article Title: miR-543 promotes colorectal cancer proliferation and metastasis by targeting KLF4

doi: 10.18632/oncotarget.19495

Figure Lengend Snippet: (A) KLF4 protein expression was detected in HCT116 cells co-transfected with miR-543 inhibitor/miR-NC and KLF4 siRNA or si-NC. (B–E) Cell proliferation, apoptosis, migration and invasion were assessed in HCT116 cells co-transfected with miR-543 inhibitor/miR-NC and KLF4 siRNA or si-NC. * p < 0.05; the data represent the mean ± SD from triplicate measurements.

Article Snippet: Human CRC cancer cells lines HCT116, SW-480 and HT29 were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Transfection, Migration